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Murexide test for uric acid – Its principle, reagents and procedure

January 7, 2020 by Dr. Medicalstudyzone.com Leave a Comment

Murexide test for uric acid is a specific test for the determination of uric acid in blood and urine. This test is mainly used in order to help diagnose gout.

Table of Contents

  • Chemicals required:
  • Procedure:
  • Observation:
  • Precautions:

Chemicals required:

  • Concentrated HNO3
  • Dilute Ammonia solution

Procedure:

  • Take 8 drops of urine in a porcelain dish.
  • Add 2 or 3 drops of concentrated HNO3 in it.
  • Heat it in boiling water bath so that HNO3 and water evaporate leaving behind a reddish deposit.
  • Add dilute ammonia solution to it.

Observation:

The colour of the residue turns reddish-violet indicating the presence of uric acid.

Precautions:

To obtain a better result, follow the below steps.

  • Before starting the procedure, wash the apparatus before and after the experiment.
  • As usual, carefully handle all the chemicals in the laboratory.
  • Avoid urine touching with hands while doing the experiment.
  • Use fork for holding test tube.
  • The test tube used should be clean neatly and free of any dirt and chemicals because we will not get the proper result then, so try to use a clean and clear test tube for a correct result.
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Filed Under: Biochemistry Tagged With: Murexide test, Murexide test for uric acid, Murexide test for uric acid - Its principle, Murexide test for uric acid principle, reagents and procedure

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