Murexide test for uric acid is a specific test for the determination of uric acid in blood and urine. This test is mainly used in order to help diagnose gout.
Table of Contents
Chemicals required:
Concentrated HNO3
Dilute Ammonia solution
Procedure:
Take 8 drops of urine in a porcelain dish.
Add 2 or 3 drops of concentrated HNO3 in it.
Heat it in boiling water bath so that HNO3 and water evaporate leaving behind a reddish deposit.
Add dilute ammonia solution to it.
Observation:
The colour of the residue turns reddish-violet indicating the presence of uric acid.
Precautions:
To obtain a better result, follow the below steps.
Before starting the procedure, wash the apparatus before and after the experiment.
As usual, carefully handle all the chemicals in the laboratory.
Avoid urine touching with hands while doing the experiment.
Use fork for holding test tube.
The test tube used should be clean neatly and free of any dirt and chemicals because we will not get the proper result then, so try to use a clean and clear test tube for a correct result.
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