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Test for calcium, magnesium and phosphate in urine

April 8, 2017 by Dr. Medicalstudyzone.com Leave a Comment

Table of Contents

  • Test for calcium, magnesium and phosphate in urine:
    • Test for Magnesium in urine:
      • Chemicals Required:
      • Procedure:
      • Observation:
    • Test for calcium in urine:
      • Reagents:
      • Procedure:
      • Observation:
    • Test for phosphate urine:
      • Reagents:
      • Procedure:
      • Observation:
    • Note:
    • Precautions:

Test for calcium, magnesium and phosphate in urine:

So the test for calcium, magnesium and phosphate in urine can be done with ease by the following methods.

Test for Magnesium in urine:

Chemicals Required:

  • A concentrated solution of ammonia.
  • Urine

Procedure:

  • Take 5 ml of urine of urine in a test tube.
  • Add 1 ml of a concentrated solution of ammonia.
  • Heat to boil the test tube.

Observation:

A white flaky precipitate of phosphates of calcium and magnesium forms. Filter it. Discard the filtrate and dissolve the precipitate in 3 ml of 1% acetic acid.

Perform the following test on this solution for the presence of calcium and phosphate separately.

Test for calcium in urine:

Reagents:

  • 2% potassium oxalate

Procedure:

  • Take 1 ml of above solution in a test tube.
  • Add 1 ml of 2% potassium oxalate in it.
  • Mix thoroughly.

Observation:

A white precipitation of calcium oxalate is produced, indicating the presence of calcium in the urine.

Test for phosphate urine:

Reagents:

  • Ammonium molybdate

Procedure:

  • Take 1 ml of above solution in a test tube.
  • Add 1 ml of ammonium molybdate reagent.
  • Heat to boil.

Observation:

The formation of a yellow crystalline precipitate indicates the presence of phosphate in urine.

Note:

The urine on boiling evolves the CO2 which makes the reaction alkaline. This results in the formation of a precipitate of magnesium and calcium phosphates, which are re-dissolved by the addition of acetic acid. This distinguishes these salts from proteins, which on heating get coagulated and cannot be re-dissolved by the acetic acid.

Precautions:

  • Wash the apparatus before and after the experiment.
  • Carefully handle all the chemicals in the lab.
  • Avoid urine touching with hands.
  • Use forks for holding test tube.
  • The test tube used should be clean neatly and free of any dirt and chemicals because we will not get the proper result then, so try to use a clean and clear test tube for correct result.

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Filed Under: Biochemistry Tagged With: magnesium and phosphate in urine, Test for calcium, Test for calcium in urine, Test for Magnesium, Test for Magnesium in urine, Test for phosphate urine

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