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Test for phosphate in milk – Its principle and procedure

January 8, 2020 by Dr. Medicalstudyzone.com Leave a Comment

Table of Contents

  • Test for phosphate in milk:
    • Chemical required:
    • Procedure:
    • Observation:
    • Precautions:

Test for phosphate in milk:

Milk contain a high concentration of phosphates. Test for phosphate in milk is performed on the filtrate obtain from the milk. First, learn how to get filtrate from milk. To do this first take 20 ml of milk in a beaker and add 18 ml of 1% acetic acid in it. The precipitate is formed due to calcium acetate. Filter the solution to obtain precipitate and filtrate.

Now to detect phosphate in milk follow the below procedure.

Chemical required:

  • Ammonium molybdate.
  • Filtrate.

Procedure:

  • Take 1 ml of filtrate in a test tube.
  • Add 1 ml of ammonium molybdate reagent in it.
  • Boil the test tube.

Observation:

The formation of yellow crystalline precipitate indicates the presence of inorganic phosphates.

Precautions:

Follow the below steps for proper result.

  • Wash the apparatus before and after the experiment.
  • Carefully handle all the chemicals in the lab.
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Filed Under: Biochemistry Tagged With: ammonium molybdate reagent, Test for phosphate in milk, Test for phosphate in milk - Its principle and procedure

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