Millon's test: (Test For Detection of Tyrosine) Significance: Amino Acid Tyrosine (Hydroxyphenylalanine) and other phenolic compounds give this reaction. Millon's test is used for the detection of amino acid tyrosine. REAGENTS: Millon's Reagent: Mixture of mercurous and mercuric nitrates. PRINCIPLE: The mercurous and mercuric nitrate reacts with the hydroxybenzene radicals … [Read more...]
Protein Biuret test – Its Principle, Reagents, Procedure etc
What is Protein Biuret test: Protein Biuret test is given by all substances containing two or more peptide linkages i.e. proteins and their hydrolytic products (proteases and Peptones). Dipeptides and amino acids do not give this reaction. The name "Biuret" was given to a compound Which was produced after urea was heated at 180°C. This compound on reaction With the dilute … [Read more...]
Ninhydrin test – Its Principle, Reagents, Procedure etc
Ninhydrin test: ( Triketo Hydrindene Hydrate) Both free and combined α-amino acids give Ninhydrin test. Significances: Ninhydrin test is used for the detection of amino acids in a solution. Ninhydrin When reacts with ammonia forms a dark blue or purple colour known as Ruhemann's purple. So amino acid detection a solution mainly occurs by performing ninhydrin test. REAGENTS: … [Read more...]
Hay’s test for bile salts – Its principle, reagent and procedure
Hay's test for bile salts: Hay's test for bile salts is a specific test used for the qualitative detection of bile salts in urine. Bile salt appears in the urine of patients suffering from jaundice. It consists of a watery mixture of organic and inorganic compounds. Bile acids are cholic acid and chenodeoxycholic acid which form conjugation with glycine and taurine. These two … [Read more...]
Rothera’s test by Nitroprusside reaction – Its principle and procedure
Rothera's test by Nitroprusside reaction: Rothera's test by Nitroprusside reaction is a specific test used for the determination of ketone bodies. The following ketones bodies or acetone bodies are found in the urine during a condition called "ketosis." Acetone. Acetoacetic acid. β- Hydroxybutyric acid. Ketosis may be associated with diabetes mellitus called Diabetic … [Read more...]