Medical Study Zone

Guides And Notes For Medical Students

  • Home
  • First Year MBBS
  • 2nd Year MBBS
  • 3rd Year MBBS
  • Final Year MBBS
  • Medical Books
    • Internal Medicine
      • CARDIOLOGY
      • NEUROLOGY
      • PEDIATRICS
      • RADIOLOGY
      • ENDOCRINOLOGY
      • ENT
      • GASTROENTEROLOGY
      • PULMONOLOGY
      • DERMATOLOGY
      • EMERGENCY MEDICINE
      • SPORTS MEDICINE
      • OPHTHALMOLOGY
      • HEMATOLOGY
      • ONCOLOGY
      • ORTHOPEDICS
      • PSYCHIATRY
      • INFECTIOUS DISEASES
      • IMMUNOLOGY
    • Anatomy
    • Biochemistry
    • Physiology
    • Forensic Medicine
    • SURGERY
      • Urology
      • Neurosurgery
      • ORTHOPEDICS
    • Pathophysiology
    • NEONATOLOGY
    • EMBRYOLOGY
    • MICROBIOLOGY
    • Histology
    • Pathology
    • Pharmacology
    • Community Medicine
    • VETERINARY SCIENCE
    • Notes
  • IELTS
  • DENTISTRY BOOKS
    • OPERATIVE DENTISTRY
    • ORAL AND MAXILLOFACIAL SURGERY
    • ORAL PATHOLOGY
    • ORTHODONTICS
    • PERIODONTOLOGY
    • PROSTHODONTICS
  • Nursing Books
  • ALL MBBS Books PDF 2023 – [First Year to Final Year] Free Download
  • USMLE
    • USMLE STEP 1
    • USMLE STEP 2
      • USMLE STEP 2 CK
      • USMLE STEP 2 CS
    • USMLE STEP 3
  • PLAB
    • PLAB 1
    • PLAB 2
  • FCPS
    • FCPS Part 1
    • FCPS Part 2
  • NEET
  • NCLEX
  • NBME
  • MRCOG
  • MRCP
  • MRCS
  • NBDE BOOKS
    • NBDE Part 1 Books
    • NBDE Part 2 Books
  • VIDEO LECTURES
  • Disclaimer
  • Privacy Policey
  • Contact
  • About us

Pauly’s test – Its principle, reagent and procedure

January 3, 2020 by Dr. Medicalstudyzone.com Leave a Comment

Table of Contents

  • Pauly’s test:
  • Pauly’s Test Principle:
  • Pauly’s test Reagent:
  • Pauly’s Test Procedure:
  • Observation:
  • Precautions:

Pauly’s test:

Pauly’s test is a specific test used for the qualitative detection of tyrosine or histidine (imidazole ring). It is named after German chemist Hermann Pauly, who first described the reaction.

Pauly’s Test Principle:

Diazotised sulphanilic acid reacts with imidazole ring in an alkaline medium to form a red coloured complex.

Pauly’s test Reagent:

  • Sulphanilic acid dissolved in hydrochloric acid.

Pauly’s Test Procedure:

  • Take 2 ml of original solution (Protein solution) in a test tube.
  • Add few drops of diazotized sulphanilic acid in it.
  • Also, add 1 ml of 20% Na2CO3 to the test tube.

Observation:

When proteins containing tyrosine or histidine reacts with diazotised sulphanilic acid under alkaline conditions, a red colour complex is formed by coupling reaction which indicates the presence of tyrosine or histidine.

Pauly's test

Precautions:

For a better result, follow the below steps.

  • Before start performing experiment make sure to wash the apparatus before and then after the experiment.
  • Carefully handle all the chemicals in the laboratory.
  • Use test tube holder, if necessary for holding test tube.
  • The test tube used should be clean neatly and free of any dirt and chemicals because we will not get the proper result then, so try to use a clean and clear test tube for a correct result.
  • After experiment completes, place the apparatus in their respective place.

 

Related Posts More From Author:

  • German For Dummies 2nd Edition by Anne Fox PDF Free Download
    German For Dummies 2nd Edition by Anne Fox PDF Free Download
  • Millon's test - Its Principle, Reagents, Procedure etc
    Millon's test - Its Principle, Reagents, Procedure etc
  • All Protein Precipitation tests with Proper Procedures
    All Protein Precipitation tests with Proper Procedures
  • Clinical Quiz Series For Medical Students And Board Candidates 2021 PDF Free Download
    Clinical Quiz Series For Medical Students And Board…
  • Xanthoproteic test for detection of aromatic amino acids
    Xanthoproteic test for detection of aromatic amino acids
  • 501 German Verbs Fifth Edition PDF Free Download
    501 German Verbs Fifth Edition PDF Free Download
  • Board and Beyond USMLE STEP 1 2023 Videos And PDF Free Download
    Board and Beyond USMLE STEP 1 2023 Videos And PDF Free…
  • Protein Biuret test - Its Principle, Reagents, Procedure etc
    Protein Biuret test - Its Principle, Reagents, Procedure etc

Filed Under: Biochemistry Tagged With: Diazotised sulphanilic acid, Pauly's test, Pauly's Test Principle, Pauly's Test Procedure, Pauly's test Reagent, Sulphanilic acid

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Donate Medicalstudyzone

Donate Medicalstudyzone

Guys Donate atleast 1$ and this money will be used to pay our website costs and also will buy Unique Most Demanded Books in PDF, Medical Video Lectures And Latest Qbanks and share it with you guys for FREE.

Most Important: Guys I have More than 2 TB Medical Data including Marrow videos, Prepladder V5.0 2023 videos etc. But due to my poor internet connection and server charges I am not able to upload all of them at once. Kindly Donate at least 1$ for this beautiful cause and help yourselves and others.

P.s: Our Patreon supporter will be given Instant Access to Single Mega Drive Links of (Marrow Videos, Prepladder V5.0 2023 Videos, Sktcy Vid, Dams vid, Dr Najeeb vid, Kaplan vid, BnB vid, Pathoma vid, osmosis, Armando, kenhub vid and much more) By donating just 1-5$ to Medicalstudyzone Patreon.

Categories

  • 2nd Year MBBS (4)
  • 3rd Year MBBS (2)
  • ABC BOOKS SERIES (58)
  • ACT (1)
  • Ai Bot ChatGPT (2)
  • AIIMS (12)
  • AKU Test (1)
  • AMC (5)
  • Anatomy (431)
    • Neuroanatomy (26)
  • Audio Lectures (85)
  • Behavioral Sciences (9)
  • Best Medical Products (3)
  • Biochemistry (197)
  • Biology (25)
    • Cell Biology (1)
    • Molecular Biology (1)
  • Book Series (34)
  • Chemistry (6)
  • Class 11th & 12th (60)
  • Community Medicine (86)
    • Biostatistics (1)
    • Preventive Medicine and Public Health (4)
  • Critical Care (1)
  • DENTISTRY BOOKS (586)
    • OPERATIVE DENTISTRY (17)
    • ORAL AND MAXILLOFACIAL SURGERY (34)
    • ORAL PATHOLOGY (13)
    • ORTHODONTICS (14)
    • PERIODONTOLOGY (10)
    • PROSTHODONTICS (11)
  • DHA Test (2)
  • Diabetes Mellitus (1)
  • Dialysis (1)
  • Echo (1)
  • EMBRYOLOGY (34)
  • Extra Bonus (72)
  • FCPS (84)
    • FCPS Part 1 (77)
    • FCPS Part 2 (40)
    • IMM (3)
  • Final Year MBBS (1)
  • First Year MBBS (4)
  • Flash Cards (11)
  • FMGE (13)
  • Forensic Medicine (33)
  • FRCP (1)
  • Genetics (17)
  • GMAT (4)
  • GRE EXAM (8)
  • Guideline for First Year MBBS (7)
  • Guidelines (1)
  • Health (10)
  • Histology (51)
  • Housejob (1)
  • IELTS (100)
  • IFOM (1)
  • IIT JEE (92)
  • Internal Medicine (2,363)
    • Anesthesia (56)
    • CARDIOLOGY (377)
      • Echocardiography (3)
    • DERMATOLOGY (218)
    • EMERGENCY MEDICINE (68)
    • ENDOCRINOLOGY (54)
    • ENT (44)
    • Ethics (2)
    • Family Medicine (12)
    • GASTROENTEROLOGY (67)
    • HEMATOLOGY (44)
    • Hepatology (15)
    • IMMUNOLOGY (53)
    • INFECTIOUS DISEASES (30)
    • Nephrology (32)
    • NEUROLOGY (106)
    • Obstetrics and Gynecology (147)
    • Occupational & Environmental Medicine (1)
    • ONCOLOGY (58)
    • OPHTHALMOLOGY (95)
    • ORTHOPEDICS (69)
    • PEDIATRICS (154)
    • PSYCHIATRY (68)
    • PULMONOLOGY (53)
    • RADIOLOGY (305)
    • Rheumatology (17)
    • SPORTS MEDICINE (3)
    • Urology (18)
  • Journals (4)
  • Laboratory (17)
  • Language (11)
  • MCAT (1)
  • MCCQE (3)
    • MCCQE Part 1 (3)
    • MCCQE Part 2 (3)
  • MCEM (1)
  • Medical Books (4,623)
  • Medical Entrytest Guidelines (4)
  • Medical Journals (1)
  • Medical softwares (10)
  • MICROBIOLOGY (115)
  • Mnemonics (2)
  • MRCOG (13)
  • MRCP (74)
    • MRCP Part 1 (21)
    • MRCP Part 2 (20)
  • MRCS (17)
  • NAPLEX (3)
  • NBDE BOOKS (29)
    • NBDE Part 1 Books (19)
    • NBDE Part 2 Books (13)
  • NBME (9)
  • NCLEX (178)
  • NEET (84)
  • NEET PG (53)
  • NEONATOLOGY (19)
  • Neuroscience (1)
  • Neurosurgery (1)
  • Notes (55)
  • Notespaedia (1)
  • Nursing Books (88)
  • Nutrition (13)
  • OET (1)
  • Past Question Papers (1)
  • Pathology (241)
  • Pathophysiology (13)
  • PCAT TEST (2)
  • PGMI Test (4)
  • Pharmacology (157)
  • Pharmacy (6)
  • Physical Examination (5)
  • Physics (4)
  • Physiology (144)
  • Physiotherapy (4)
  • PLAB (68)
    • PLAB 1 (46)
    • PLAB 2 (46)
  • PMC NMDCAT (92)
  • Prometric Exam Collection (2)
  • PSAT/NMSQT (1)
  • Qbanks (59)
  • Refreshment (1)
  • SAT TEST (2)
  • SURGERY (245)
    • Neurosurgery (2)
    • Paediatric Surgery (1)
    • Urology (3)
  • USMLE (579)
    • USMLE Experience (1)
    • USMLE Recalls (4)
    • USMLE STEP 1 (260)
    • USMLE STEP 2 (119)
    • USMLE STEP 2 CK (130)
    • USMLE STEP 2 CS (58)
    • USMLE STEP 3 (110)
  • UWorld (8)
  • VETERINARY SCIENCE (23)
  • VIDEO LECTURES (1,667)
  • Workshop (6)

Follow us on twitter

Follow @medicalstudyzon

Copyright © 2023 · Magazine Pro Theme on Genesis Framework · WordPress · Log in