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ARGININE TEST (Test for Guanidine Group) Its principle & procedure

April 5, 2017 by Dr. Medicalstudyzone.com Leave a Comment

Table of Contents

  • ARGININE TEST (Test for Guanidine Group):
    • Significance:
    • Reagents:
    • PROCEDURE:
    • Observation:
    • Precautions:

ARGININE TEST (Test for Guanidine Group):

Significance:

Arginine test is specific for Arginine and indicates the presence of guanidine group in the arginine molecule.

Reagents:

  • Protein solution
  • 5% sodium hydroxide
  • 1% alcoholic α-naphthol
  • sodium hypobromite (NaOBr)

PROCEDURE:

  • Take 1 ml of original solution (protein solution) in a test tube.
  • Add 1 ml of 5% sodium hydroxide to the test tube.
  • And add 2 drops of 1% alcoholic α-naphthol.
  • Mix it and add 3 drops of sodium hypobromite (NaOBr) reagent.
  • Mix it thoroughly.
  • A bright red colour is formed. This confirms the presence of arginine.

Observation:

When bright red colour forms, it mean arginine is present in the solution.

Precautions:

  • Wash the apparatus before and after the experiment.
  • Carefully handle all the chemicals while performing the experiment.

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Filed Under: Biochemistry Tagged With: 1% alcoholic α-naphthol, 5% sodium hydroxide, ARGININE TEST, ARGININE TEST (Test for Guanidine Group), ARGININE TEST (Test for Guanidine Group) Its principle & procedure

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