Test for creatinine by jaffe’s reaction – Its principle and procedure

Test for creatinine by Jaffe’s reaction is a specific test used to determine creatinine level in urine and blood. Max Jaffe first was the first who described its basic principle. He said the colour changes was directly proportional to the concentration of creatinine.

Reagents:

• Saturated picric acid.
• 5% NaOH

Procedure:

• Take 3 ml of urine in a test tube.
• Add 1 ml of saturated picric acid solution.
• And also add 1 ml of 5% NaOH.

Observations:

The development of an orange colour, due to the formation of creatinine picrate in alkaline solution, indicates the presence of creatinine in the urine.

Precautions:

Follow the below steps for a better result.

• Before starting the experiment wash the apparatus before and after the experiment.
• Carefully handle all the chemicals in the laboratory.
• Avoid urine touching with hands while doing the experiment.
• Use fork for holding test tube.
• The test tube used should be clean neatly and free of any dirt and chemicals because we will not get the proper result then, so try to use a clean and clear test tube for a correct result.

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