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Starch iodine test for polysaccharides

January 7, 2020 by Dr. Medicalstudyzone.com Leave a Comment

Starch iodine test is a specific test for polysaccharides (starch positive). Iodine test indicates the presence of polysaccharides in any solution. When iodine solution reacts with starch, a bluish colour is obtained indicating the presence of starch.

Starch iodine test
           Starch iodine test

Contents

  • 1 REAGENTS:
  • 2 PRINCIPLE:
  • 3 PROCEDURE:
  • 4 OBSERVATION AND RESULT:
  • 5 Precautions:

REAGENTS:

i. 1% Iodine solution ( which contain potassium iodide and water).

ii. Original solution (O.S) containing a carbohydrate

PRINCIPLE:

Starch, glycogen and higher dextrins on reaction with dilute iodine solution form coloured compounds.

PROCEDURE:

  • Take 2 ml of the starch solution (original solution) in a test tube.
  • Add 1-2 drops of the diluted iodine solution.
  • Observe the production of a blue colour.
  • The blue colour produced disappears on heating, and it reappears on cooling the solution.

OBSERVATION AND RESULT:

Starch, glycogen and higher dextrins on reaction with dilute iodine solution form coloured compounds as shown in the table below:

 Starch  Blue colour
 Amylodextrin  Purple colour
 Erythrodextrin  Red colour
 Glycogen  Red colour
 Achrodextrin (Lower dextrins), Disaccharides (Maltose) and Monosaccharides  No change in colour

Precautions:

  • Wash the apparatus before and after the experiment.
  • Carefully handle all the chemicals.
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Filed Under: Biochemistry Tagged With: iodine test, iodine test for Starch, Starch iodine test

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