Medical Study Zone

Guides And Notes For Medical Students

  • Home
  • First Year MBBS
  • 2nd Year MBBS
  • 3rd Year MBBS
  • Final Year MBBS
  • Medical Books
    • Internal Medicine
      • CARDIOLOGY
      • NEUROLOGY
      • PEDIATRICS
      • RADIOLOGY
      • ENDOCRINOLOGY
      • ENT
      • GASTROENTEROLOGY
      • PULMONOLOGY
      • DERMATOLOGY
      • EMERGENCY MEDICINE
      • SPORTS MEDICINE
      • OPHTHALMOLOGY
      • HEMATOLOGY
      • ONCOLOGY
      • ORTHOPEDICS
      • PSYCHIATRY
      • INFECTIOUS DISEASES
      • IMMUNOLOGY
    • Anatomy
    • Biochemistry
    • Physiology
    • Forensic Medicine
    • SURGERY
      • Urology
      • Neurosurgery
      • ORTHOPEDICS
    • Pathophysiology
    • NEONATOLOGY
    • EMBRYOLOGY
    • MICROBIOLOGY
    • Histology
    • Pathology
    • Pharmacology
    • Community Medicine
    • VETERINARY SCIENCE
    • Notes
  • IELTS
  • DENTISTRY BOOKS
    • OPERATIVE DENTISTRY
    • ORAL AND MAXILLOFACIAL SURGERY
    • ORAL PATHOLOGY
    • ORTHODONTICS
    • PERIODONTOLOGY
    • PROSTHODONTICS
  • Nursing Books
  • ALL MBBS Books PDF 2022 – [First Year to Final Year] Free Download
  • USMLE
    • USMLE STEP 1
    • USMLE STEP 2
      • USMLE STEP 2 CK
      • USMLE STEP 2 CS
    • USMLE STEP 3
  • PLAB
    • PLAB 1
    • PLAB 2
  • FCPS
    • FCPS Part 1
    • FCPS Part 2
  • NEET
  • NCLEX
  • NBME
  • MRCOG
  • MRCP
  • MRCS
  • NBDE BOOKS
    • NBDE Part 1 Books
    • NBDE Part 2 Books
  • VIDEO LECTURES
  • Disclaimer
  • Privacy Policey
  • Contact
  • About us

OSAZONE TEST – For differentiation of reducing sugar

January 6, 2020 by Dr. Medicalstudyzone.com Leave a Comment

Osazone test is performed to differentiate the reducing sugars on the basis of crystal formation.

Table of Contents

  • REAGENTS:
  • PRINCIPLE:
  • PROCEDURE:
  • NOTE:
  • Observation and Result:
  • Precautions:

REAGENTS:

1. Phenylhydrazine mixture consisting of:

i. Phenylhydrazine, and

ii. Anhydrous sodium acetate.

2. Original solution (0.8.) – containing a carbohydrate

PRINCIPLE:

Aldehyde or keto group containing sugars reacts with the amine group of phenylhydrazine to give phenylosazone.

PROCEDURE:

  • Take phenylhydrazine mixture and till about half an inch of the test tube with it.
  • Add 3 to 5ml of sugar solution (original solution) to the test tube.
  • Mix thoroughly and place it in a boiling water bath.
  • Note the appearance of a yellow crystalline precipitate in the test tube.
  • Remove and allow the test tube to cool slowly.
  • Do not cool the test tube under tap water.
  • Examine the crystals under the microscope and make a drawing.
  • Draw only what you see.

NOTE:

Osazones of glucose are formed in 10-15 minutes whereas those of disaccharides take up to 45 minutes. Sucrose does not react with phenylhydrazine to form crystalline compounds called osazones.

Observation and Result:

SUGAR  OSAZONE  SHAPE
 Glucose   Glucosazone  Needle-shaped
 Fructose   Fructosazone  Needle-shaped
 Galactose  Galactosazone  Fluffy ball shaped
 Lactose   Lactosazone  Fluffy ball shaped
 Maltose   Maltosazone  Sunflower shaped

 

OSAZONE TEST
          OSAZONE TEST

Precautions:

  • Wash the apparatus before and after the experiment.
  • Carefully Observe the Crystals under the microscope.
  • Carefully handle all the chemicals.
  • Test for calcium in milk – Its principle and procedure
  • Test for Chlorides in milk – Its principle and procedure
  • Test for calcium, magnesium and phosphate in urine
  • Salkowski test for cholesterol – Its principle and procedure
  • Test for Chlorides in urine – Its principle and procedure
  • Foam test for bile salts and bile pigments
  • Test for Lactalbumin and Lactoglobulin in milk – Its principle and procedure

Related Posts More From Author:

  • 10 Best Stethoscopes to buy (2023) - Buyer's guide and Review
    10 Best Stethoscopes to buy (2023) - Buyer's guide and…
  • Benedict's test for reducing sugar
    Benedict's test for reducing sugar
  • All Protein Precipitation tests with Proper Procedures
    All Protein Precipitation tests with Proper Procedures
  • Barfoed's test for mono and disaccharides
    Barfoed's test for mono and disaccharides
  • Xanthoproteic test for detection of aromatic amino acids
    Xanthoproteic test for detection of aromatic amino acids
  • Seliwanoff's test for aldo and keto sugar
    Seliwanoff's test for aldo and keto sugar
  • Test for phosphate in milk - Its principle and procedure
    Test for phosphate in milk - Its principle and procedure
  • Method Determine of Erythrocyte Sedimentation Rate (ESR) 
    Method Determine of Erythrocyte Sedimentation Rate (ESR) 

Filed Under: Biochemistry Tagged With: differentiation of reducing sugar, OSAZONE TEST, OSAZONE TEST For differentiation of reducing sugar

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Donate Medicalstudyzone

Donate Medicalstudyzone

Guys Donate atleast 1$ and this money will be used to pay our website costs and also will buy Unique Most Demanded Books in PDF, Medical Video Lectures And Latest Qbanks and share it with you guys for FREE.

Most Important: Guys I have More than 2 TB Medical Data including Marrow videos, Prepladder videos etc. But due to my poor internet connection and server charges I am not able to upload all of them at once. Kindly Donate at least 1$ for this beautiful cause and help yourselves and others.

P.s: Our Patreon supporter will be given access to prep and mrw video on telegram instantly upon donation of 1-5$ to Medicalstudyzone Patreon

Categories

  • 2nd Year MBBS (3)
  • 3rd Year MBBS (2)
  • ABC BOOKS SERIES (58)
  • ACT (1)
  • Ai Bot ChatGPT (2)
  • AIIMS (10)
  • AMC (5)
  • Anatomy (427)
    • Neuroanatomy (26)
  • Audio Lectures (85)
  • Behavioral Sciences (9)
  • Best Medical Products (3)
  • Biochemistry (195)
  • Biology (24)
    • Cell Biology (1)
    • Molecular Biology (1)
  • Book Series (34)
  • Chemistry (3)
  • Class 11th & 12th (56)
  • Community Medicine (85)
    • Preventive Medicine and Public Health (4)
  • Critical Care (1)
  • DENTISTRY BOOKS (586)
    • OPERATIVE DENTISTRY (17)
    • ORAL AND MAXILLOFACIAL SURGERY (34)
    • ORAL PATHOLOGY (13)
    • ORTHODONTICS (14)
    • PERIODONTOLOGY (10)
    • PROSTHODONTICS (11)
  • Diabetes Mellitus (1)
  • Dialysis (1)
  • Echo (1)
  • EMBRYOLOGY (33)
  • Extra Bonus (72)
  • FCPS (83)
    • FCPS Part 1 (77)
    • FCPS Part 2 (39)
    • IMM (2)
  • Final Year MBBS (1)
  • First Year MBBS (4)
  • Flash Cards (11)
  • Forensic Medicine (32)
  • FRCP (1)
  • Genetics (17)
  • GMAT (4)
  • GRE EXAM (8)
  • Guideline for First Year MBBS (7)
  • Health (10)
  • Histology (51)
  • Housejob (1)
  • IELTS (100)
  • IFOM (1)
  • IIT JEE (87)
  • Internal Medicine (2,326)
    • Anesthesia (56)
    • CARDIOLOGY (375)
      • Echocardiography (3)
    • DERMATOLOGY (218)
    • EMERGENCY MEDICINE (66)
    • ENDOCRINOLOGY (52)
    • ENT (44)
    • Ethics (1)
    • Family Medicine (12)
    • GASTROENTEROLOGY (65)
    • HEMATOLOGY (42)
    • Hepatology (13)
    • IMMUNOLOGY (52)
    • INFECTIOUS DISEASES (28)
    • Nephrology (30)
    • NEUROLOGY (104)
    • Obstetrics and Gynecology (146)
    • Occupational & Environmental Medicine (1)
    • ONCOLOGY (58)
    • OPHTHALMOLOGY (95)
    • ORTHOPEDICS (68)
    • PEDIATRICS (154)
    • PSYCHIATRY (67)
    • PULMONOLOGY (51)
    • RADIOLOGY (302)
    • Rheumatology (15)
    • SPORTS MEDICINE (3)
    • Urology (18)
  • Journals (4)
  • Laboratory (17)
  • Language (11)
  • MCAT (1)
  • MCEM (1)
  • Medical Books (4,596)
  • Medical Entrytest Guidelines (4)
  • Medical Journals (1)
  • Medical softwares (9)
  • MICROBIOLOGY (111)
  • Mnemonics (2)
  • MRCOG (12)
  • MRCP (68)
    • MRCP Part 1 (15)
    • MRCP Part 2 (14)
  • MRCS (16)
  • NAPLEX (3)
  • NBDE BOOKS (29)
    • NBDE Part 1 Books (19)
    • NBDE Part 2 Books (13)
  • NBME (9)
  • NCLEX (178)
  • NEET (71)
  • NEET PG (9)
  • NEONATOLOGY (19)
  • Neuroscience (1)
  • Neurosurgery (1)
  • Notes (26)
  • Notespaedia (1)
  • Nursing Books (88)
  • Nutrition (13)
  • OET (1)
  • Pathology (236)
  • Pathophysiology (12)
  • PCAT TEST (2)
  • Pharmacology (157)
  • Pharmacy (6)
  • Physical Examination (5)
  • Physics (2)
  • Physiology (142)
  • Physiotherapy (4)
  • PLAB (66)
    • PLAB 1 (44)
    • PLAB 2 (44)
  • PMC NMDCAT (92)
  • PSAT/NMSQT (1)
  • Qbanks (58)
  • Refreshment (1)
  • SAT TEST (2)
  • SURGERY (242)
    • Neurosurgery (2)
    • Paediatric Surgery (1)
    • Urology (3)
  • USMLE (561)
    • USMLE Recalls (4)
    • USMLE STEP 1 (243)
    • USMLE STEP 2 (118)
    • USMLE STEP 2 CK (129)
    • USMLE STEP 2 CS (57)
    • USMLE STEP 3 (108)
  • UWorld (8)
  • VETERINARY SCIENCE (23)
  • VIDEO LECTURES (1,646)
  • Workshop (6)

Follow us on twitter

Follow @medicalstudyzon

Copyright © 2023 · Magazine Pro Theme on Genesis Framework · WordPress · Log in