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OSAZONE TEST – For differentiation of reducing sugar

January 6, 2020 by Dr. Medicalstudyzone.com Leave a Comment

Osazone test is performed to differentiate the reducing sugars on the basis of crystal formation.

Table of Contents

  • REAGENTS:
  • PRINCIPLE:
  • PROCEDURE:
  • NOTE:
  • Observation and Result:
  • Precautions:

REAGENTS:

1. Phenylhydrazine mixture consisting of:

i. Phenylhydrazine, and

ii. Anhydrous sodium acetate.

2. Original solution (0.8.) – containing a carbohydrate

PRINCIPLE:

Aldehyde or keto group containing sugars reacts with the amine group of phenylhydrazine to give phenylosazone.

PROCEDURE:

  • Take phenylhydrazine mixture and till about half an inch of the test tube with it.
  • Add 3 to 5ml of sugar solution (original solution) to the test tube.
  • Mix thoroughly and place it in a boiling water bath.
  • Note the appearance of a yellow crystalline precipitate in the test tube.
  • Remove and allow the test tube to cool slowly.
  • Do not cool the test tube under tap water.
  • Examine the crystals under the microscope and make a drawing.
  • Draw only what you see.

NOTE:

Osazones of glucose are formed in 10-15 minutes whereas those of disaccharides take up to 45 minutes. Sucrose does not react with phenylhydrazine to form crystalline compounds called osazones.

Observation and Result:

SUGAR  OSAZONE  SHAPE
 Glucose   Glucosazone  Needle-shaped
 Fructose   Fructosazone  Needle-shaped
 Galactose  Galactosazone  Fluffy ball shaped
 Lactose   Lactosazone  Fluffy ball shaped
 Maltose   Maltosazone  Sunflower shaped

 

OSAZONE TEST
          OSAZONE TEST

Precautions:

  • Wash the apparatus before and after the experiment.
  • Carefully Observe the Crystals under the microscope.
  • Carefully handle all the chemicals.
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Filed Under: Biochemistry Tagged With: differentiation of reducing sugar, OSAZONE TEST, OSAZONE TEST For differentiation of reducing sugar

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