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Seliwanoff’s test for aldo and keto sugar

January 1, 2020 by Dr. Medicalstudyzone.com 2 Comments

Seliwanoff’s test is used to differentiate between keto sugar and aldo sugar.

Table of Contents

  • PRINCIPLE:
  • REAGENTS:
  • PROCEDURE:
  • INDICATION:
  • RESULT:
  • NOTE:
  • Precautions:

PRINCIPLE:

Carbohydrates are converted to furfural derivatives by dilute HCl. Only furfural derivatives of keto-hexoses condense with Resorcinol to form a cherry red complex. This test is positive for sugar (Keto) when it gives cherry red colour complex.

REAGENTS:

1. Seliwanoff’s Reagent: i. Resorcinol, and ii. Concentrated HCl. 2. Original solution (0.8.) containing a carbohydrate

PROCEDURE:

  • Take 3ml of seliwanoff’s reagent in a test tube.
  • Add 5ml of original solution.
  • Heat the test tube just to boil.
  • Avoid prolonged heating otherwise; it gives a false test.

INDICATION:

The production of a Red colour indicates a positive reaction.

RESULT:

  • No change in colour = Aldo sugar
  • If cherry red colour appears = Keto sugar is present.

NOTE:

Avoid prolonged heating. Prolong boiling with aldoses is bound to give a false positive result, as the aldoses also start giving a similar reaction.

Precautions:

  • Wash the apparatus before and after the experiment.
  • Avoid prolonged heating as it will then gives a false result.

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Filed Under: Biochemistry Tagged With: Seliwanoff's test

Comments

  1. mohammed says

    May 8, 2018 at 7:26 pm

    NICE

    Reply
    • Dr. Arshad Bangash says

      May 15, 2018 at 2:59 pm

      check inbox

      Reply

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