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Liebermann burchard test for cholesterol – Its principle and procedure

April 5, 2017 by Dr. Medicalstudyzone.com Leave a Comment

Table of Contents

  • Liebermann Burchard test for cholesterol:
    • Reagents:
    • Procedure:
    • Observations:
    • Precautions:

Liebermann Burchard test for cholesterol:

Liebermann Burchard test is a specific test for the detection of cholesterol.

Reagents:

  • Cholesterol solution
  • Acetic anhydride
  • Concentrated sulphuric acid

Procedure:

  • Take 1 ml of cholesterol solution in a dry test tube.
  • Add 10 drops of acetic anhydride.
  • Then add 2 drops of concentrated sulphuric acid.
  • Mix well.

Observations:

The solution becomes red which rapidly changes to violet, then to deep blue and finally, on standing, it changes to deep bluish green colour.

Precautions:

  • Before starting the experiment wash the apparatus cleanly.
  • Carefully handle the sulphuric acid and all others chemicals in the lab.

 

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Filed Under: Biochemistry Tagged With: Liebermann burchard, Liebermann burchard test, Liebermann burchard test for cholesterol -, Liebermann burchard test for cholesterol - Its principle and procedure, Liebermann test

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