Test for calcium in milk: Milk contain a high concentration of calcium. Test for calcium in milk is performed on the filtrate obtain from the milk. First, learn how to get filtrate from milk. To do this first take 20 ml of milk in a beaker and add 18 ml of 1% acetic acid in it. The precipitate is formed due to calcium acetate. Filter the solution to obtain precipitate and … [Read more...]
Archives for April 2017
Test for Lactalbumin and Lactoglobulin in milk – Its principle and procedure
Test for Lactalbumin and Lactoglobulin in milk: To perform the test for Lactalbumin and Lactoglobulin in milk, first we will extract filtrate of milk, and then we will carry out the test on this filtrate. To obtain filtrate of milk follows the below procedure. Obtaining of filtrate from milk: Procedure: Take 20 ml of milk in a beaker. Add 1% acetic acid drop by … [Read more...]
Fat determination in milk – Its principle and procedure
Test for Fat determination in milk: Milk is the fluid secreted by the mammary gland, particularly free from colostrum. It is an emulsion of lipids in a solution of proteins, a sugar, and inorganic salts. Other constituents include some organic acids or their salts, vitamins, enzymes and antibodies. We will explain fat determination in milk below. Precipitation of Casein and … [Read more...]
Liebermann burchard test for cholesterol – Its principle and procedure
Liebermann Burchard test for cholesterol: Liebermann Burchard test is a specific test for the detection of cholesterol. Reagents: Cholesterol solution Acetic anhydride Concentrated sulphuric acid Procedure: Take 1 ml of cholesterol solution in a dry test tube. Add 10 drops of acetic anhydride. Then add 2 drops of concentrated sulphuric acid. Mix … [Read more...]
Salkowski test for cholesterol – Its principle and procedure
Salkowski test for cholesterol: Salkowski test is used to detect cholesterol in a solution. This test is named after a German biochemist Ernst Leopold Salkowski. Reagents: Cholesterol solution Chloroform Concentrated Sulphuric acid Procedure: Take 2 ml of cholesterol solution, in chloroform. Add 2 ml of concentrated sulphuric acid. Shake … [Read more...]
General tests for lipids – Its principle and procedure
General tests for lipids: Lipids comprise of very important group of organic substances present in plant and animal tissues. They have low melting point and are usually liquid at body temperature. They have high energy value. Lipids include fats, oils, steroids, waxes and other related compunds. They are insoluble in water but are soluble to varying degree in organic solvents … [Read more...]
ARGININE TEST (Test for Guanidine Group) Its principle & procedure
ARGININE TEST (Test for Guanidine Group): Significance: Arginine test is specific for Arginine and indicates the presence of guanidine group in the arginine molecule. Reagents: Protein solution 5% sodium hydroxide 1% alcoholic α-naphthol sodium hypobromite (NaOBr) PROCEDURE: Take 1 ml of original solution (protein solution) in a test tube. Add 1 ml … [Read more...]
Ammonium sulphate saturation test – Its principle, reagents and procedure
Ammonium sulphate saturation test: Significance: Ammonium sulphate saturation test is a confirmatory test. Half saturation (50% saturation) with ammonium sulphate will result in precipitation of globulins, whereas albumins are precipitated after full saturation (100% saturation) of the test sample with ammonium. Rragents: Ammonium sulphate crystals. Principle: Protein is … [Read more...]