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Archives for April 2017

Method determination of red blood cell (RBC) count

April 16, 2017 by Dr. Arshad Bangash Leave a Comment

Method determination of red blood cell (RBC) count

Method determination of red blood cell (RBC) count: APPARATUS: Neubauer’s chamber (thick slide), RBC diluting fluid, microscope, coverslip, pricking needle (blood Lancet) and spirit swab. RBC Diluting Fluid (Hayem’s Fluid) Sodium chloride (NaCl): 0.5 gm, to maintain isotonicity of fluid. Sodium sulphate (Na2SO4): 2.5 gm, which breaks RBCs and prevents their rouleaux … [Read more...]

Haemocytometer – Improved neubauer’s chamber

April 14, 2017 by Dr. Arshad Bangash Leave a Comment

Haemocytometer - Improved neubauer's chamber

Haemocytometer - Improved Neubauer's chamber: The Haemocytometer: Haemocytometer is an apparatus used to count various blood cells (RBS, WBC, Eosinophil and platelets). It consists of RBC, and WBC pipette ( Thoma diluting pipettes) and a thick slide (Neubauer's Chamber). Reagents: Hayem's fluid for RBC counting and its composition: NaCl - 0.5 g for isotonicity. … [Read more...]

Methods determination of bleeding time vs clotting time of blood

April 13, 2017 by Dr. Arshad Bangash Leave a Comment

Methods determination of bleeding time vs clotting time of blood

Methods Determination of bleeding time vs clotting time of blood: DEFINITIONS: A. Bleeding time: It is the time taken for stoppage of bleeding from the site of skin puncture due to the formation of the haemostatic plug. B. Clotting time: It is time taken by the clotting factors of the blood to start haemostasis and the production of a proteinous web of fibrin thread to … [Read more...]

Foam test for bile salts and bile pigments

April 12, 2017 by Dr. Arshad Bangash Leave a Comment

Foam test for bile salts and bile pigments

Foam test for bile salts and bile pigments: Foam test for bile salts and bile pigments is a specific test used for the qualitative detection of bile salts and bile pigments in urine. There are other test as well which can be used to detect bile salts and bile pigments in urine. These tests are Gmelin's test for bile pigments and Hay's test for bile salts respectively. Foam … [Read more...]

Gmelin’s test for bile pigments – Its principle, reagent and procedure

April 12, 2017 by Dr. Arshad Bangash 1 Comment

Gmelin's test

Gmelin's test for bile pigments: Gmelin's test is a specific test used for the qualitative detection of bile pigments in urine. The bile pigments i.e bilirubin and biliverdin are almost entirely excreted through the bile by the liver but in conditions like obstructive jaundice, where bile ducts get blocked, the bile pigments start appearing in the urine. Gmelin's test … [Read more...]

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Arshad Bangash

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Arshad Bangash is a Final year Medical student at Ayub Medical College, Abbottabad Pakistan. Besides medicine, he has a great passion for Cooking food, Video Gaming and blogging. He is the founder/administrator of Medical Study Zone. He blogs to help others and share his knowledge with others.

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