Method determination of red blood cell (RBC) count: APPARATUS: Neubauer’s chamber (thick slide), RBC diluting fluid, microscope, coverslip, pricking needle (blood Lancet) and spirit swab. RBC Diluting Fluid (Hayem’s Fluid) Sodium chloride (NaCl): 0.5 gm, to maintain isotonicity of fluid. Sodium sulphate (Na2SO4): 2.5 gm, which breaks RBCs and prevents their rouleaux … [Read more...]
Archives for April 2017
Haemocytometer – Improved neubauer’s chamber
Haemocytometer - Improved Neubauer's chamber: The Haemocytometer: Haemocytometer is an apparatus used to count various blood cells (RBS, WBC, Eosinophil and platelets). It consists of RBC, and WBC pipette ( Thoma diluting pipettes) and a thick slide (Neubauer's Chamber). Reagents: Hayem's fluid for RBC counting and its composition: NaCl - 0.5 g for isotonicity. … [Read more...]
Methods determination of bleeding time vs clotting time of blood
Methods Determination of bleeding time vs clotting time of blood: DEFINITIONS: A. Bleeding time: It is the time taken for stoppage of bleeding from the site of skin puncture due to the formation of the haemostatic plug. B. Clotting time: It is time taken by the clotting factors of the blood to start haemostasis and the production of a proteinous web of fibrin thread to … [Read more...]
Foam test for bile salts and bile pigments
Foam test for bile salts and bile pigments: Foam test for bile salts and bile pigments is a specific test used for the qualitative detection of bile salts and bile pigments in urine. There are other test as well which can be used to detect bile salts and bile pigments in urine. These tests are Gmelin's test for bile pigments and Hay's test for bile salts respectively. Foam … [Read more...]
Gmelin’s test for bile pigments – Its principle, reagent and procedure
Gmelin's test for bile pigments: Gmelin's test is a specific test used for the qualitative detection of bile pigments in urine. The bile pigments i.e bilirubin and biliverdin are almost entirely excreted through the bile by the liver but in conditions like obstructive jaundice, where bile ducts get blocked, the bile pigments start appearing in the urine. Gmelin's test … [Read more...]