Pauly's test: Pauly's test is a specific test used for the qualitative detection of tyrosine or histidine (imidazole ring). It is named after German chemist Hermann Pauly, who first described the reaction. Pauly's Test Principle: Diazotised sulphanilic acid reacts with imidazole ring in an alkaline medium to form a red coloured complex. Pauly's test Reagent: … [Read more...]
Archives for April 2017
Method determination of red blood cell (RBC) count
Method determination of red blood cell (RBC) count: APPARATUS: Neubauer’s chamber (thick slide), RBC diluting fluid, microscope, coverslip, pricking needle (blood Lancet) and spirit swab. RBC Diluting Fluid (Hayem’s Fluid) Sodium chloride (NaCl): 0.5 gm, to maintain isotonicity of fluid. Sodium sulphate (Na2SO4): 2.5 gm, which breaks RBCs and prevents their rouleaux … [Read more...]
Haemocytometer – Improved neubauer’s chamber
Haemocytometer - Improved Neubauer's chamber: The Haemocytometer: Haemocytometer is an apparatus used to count various blood cells (RBS, WBC, Eosinophil and platelets). It consists of RBC, and WBC pipette ( Thoma diluting pipettes) and a thick slide (Neubauer's Chamber). Reagents: Hayem's fluid for RBC counting and its composition: NaCl - 0.5 g for isotonicity. … [Read more...]
Methods determination of bleeding time vs clotting time of blood
Methods Determination of bleeding time vs clotting time of blood: DEFINITIONS: A. Bleeding time: It is the time taken for stoppage of bleeding from the site of skin puncture due to the formation of the haemostatic plug. B. Clotting time: It is time taken by the clotting factors of the blood to start haemostasis and the production of a proteinous web of fibrin thread to … [Read more...]
Gerhard’s test for ketone bodies – Its principle and procedure
Gerhard's test: Gerhard's test is a specific test used for the qualitative detection of ketone bodies in urine. The following ketones bodies or acetone bodies are found in the urine during a condition called “ketosis.” Acetone. Acetoacetic acid. β- Hydroxybutyric acid. These are synthesized in liver during starvation and uncontrolled diabetes. Ketone bodies are … [Read more...]