Test for organic sulphates in urine:

Test for organic sulphates in urine is a common test used for the detection of sulphates in urine and blood.

Reagents:

• Concentrated HCL
• 10% barium chloride

Procedure:

• Take 3 ml of urine in a test tube.
• Add 1 ml concentrated HCL in it.
• And 1 ml 10% barium chloride.
• Mix thoroughly.
• A white precipitate form.
• Filter it to obtain a clear filtrate.
• Heat the filtrate in a boiling water bath for 3 minutes.

Observation:

A faint white cloud of barium sulphate is produced, indicating the presence of organic sulphates.

Precautions:

To obtain a better result, follow the below steps.

• Before starting the procedure, wash the apparatus before and after the experiment.
• As usual, carefully handle all the chemicals in the laboratory.
• Avoid urine touching with hands while doing the experiment.
• Use fork for holding test tube.
• The test tube used should be clean neatly and free of any dirt and chemicals because we will not get the proper result then, so try to use a clean and clear test tube for a correct result.