Urease test for Urea:

So the urease test for Urea is a specific test for the detection of urea in urine.

Urease test principle:

Reagents:

• Phenol red
• Urease solution

Procedure:

• Take 2 ml of urine in a test tube.
• Add 2 drops of phenol red in it.
• And 1 ml of urease solution.
• Mix well.
• Now incubate it for 15 minutes at 37 ºC.

Observation:

The solution turns red due to the conversion of urea into ammonia.

Precautions:

Follow the below steps for a better result.

• Wash the apparatus before and after the experiment.
• Carefully handle all the chemicals in the lab.
• Avoid urine touching with hands.
• Use forks for holding test tube.
• The test tube used should be clean neatly and free of any dirt and chemicals because we will not get the proper result then, so try to use a clean and clear test tube for a correct result.