Murexide test for uric acid is a specific test for the determination of uric acid in blood and urine. This test is mainly used in order to help diagnose gout. Chemicals required: Concentrated HNO3 Dilute Ammonia solution Procedure: Take 8 drops of urine in a porcelain dish. Add 2 or 3 drops of concentrated HNO3 in it. Heat it in boiling water bath so that … [Read more...]
Cysteine test for sulphur – Its principle, reagents and procedure
Cysteine test for sulphur: Significance: Cysteine test for sulphur is used for the detection of sulphur containing amino acids. Reagents: Sodium hydroxide (NaOH) Lead Acetate Principle: Protein or amino acids when heated with sodium hydroxide, sulphur splits out as sodium sulphide, which on reaction with lead acetate, forms grayish brown to black precipitate of … [Read more...]
Benzidine test – Its principle, reagents and procedure
Benzidine test: Benzidine test is a specific test used for the determination of blood (RBCs or haemoglobin) in urine. Kidney diseases or any injury to the urinary tract may result in leakage of blood in the urine. Blood (Haemoglobin) may occur in the urine as intact corpuscles (RBC) as in haematuria or free in the solution (Haemoglobin). Haemoglobinuria occurs in a certain … [Read more...]
Indican test in urine – Its principle, reagents and procedure
Indican test in urine: Indican test in urine is a general test used for the determination of indican in urine. The Indican test in urine is also called an obermeyer test. Indican is an indole which is produced when intestinal bacteria in the intestine act on the amino acid, tryptophan. Reagents: 1% alcoholic α-naphthol 3% H2O2 Concentrated HCL Procedure: … [Read more...]
Urease test for Urea – Its principle, reagents and procedure
Urease test for Urea: So the urease test for Urea is a specific test for the detection of urea in urine. Urease test principle: Urea is a diamide of carbonic acid. Urea is hydrolyzed with the release of ammonia and carbon dioxide. Many organisms especially have an urease enzyme which can split urea in the presence of water to release ammonia and carbon dioxide. The ammonia … [Read more...]
Ammonium sulphate saturation test – Its principle, reagents and procedure
Ammonium sulphate saturation test: Significance: Ammonium sulphate saturation test is a confirmatory test. Half saturation (50% saturation) with ammonium sulphate will result in precipitation of globulins, whereas albumins are precipitated after full saturation (100% saturation) of the test sample with ammonium. Rragents: Ammonium sulphate crystals. Principle: Protein is … [Read more...]