Osazone test is performed to differentiate the reducing sugars on the basis of crystal formation. REAGENTS: 1. Phenylhydrazine mixture consisting of: i. Phenylhydrazine, and ii. Anhydrous sodium acetate. 2. Original solution (0.8.) – containing a carbohydrate PRINCIPLE: Aldehyde or keto group containing sugars reacts with the amine group of phenylhydrazine to give … [Read more...]