Tryptophan is an amino acid. Hopkins Cole test is a specific test used for the detection of tryptophan. When tryptophan is present in a solution, it gives a reddish violet ring. REAGENTS of Hopkins Cole test: Hopkins Cole Reagent: Magnesium compound of Glyoxalic acid and concentrated H2SO4. PRINCIPLE: Concentrated H2SO4 causes dehydration of tryptophan which reacts with … [Read more...]
All Protein Precipitation tests with Proper Procedures
PROTEIN PRECIPITATION TESTS: Protein gives protein precipitation tests because Proteins behave as cations in acid solutions and are precipitated with acid radicals to form insoluble salts. On the other hand, in alkaline solutions, they act as anions and are precipitated with heavy metals.By virtue of their sulphydryl (-SH) groups, they also act with salts of heavy … [Read more...]